ที่มา:วารสาร คณะสัตวแพทยศาสตร์
หัวเรื่อง:ไม่มีชื่อไทย (ชื่ออังกฤษ : Comparison of Serological Tests for Antibody Detection Against Brucella melitensis Infection in Goats)
ผู้เขียน:
Monaya Ekgatat, Reka Kanitpun, Pittaya Kunchit, Worawan Arampong, Sornsak Raksajit, Suree Thammasart, Utit Trenuntawan, Phairuch Tumcha, Surapong Wongkasemjit
สื่อสิ่งพิมพ์:pdf
Abstract
Four serological assays, including Rose Bengal test (RBT), modified RBT (mRBT), complement fixation test (CET) and indirect enzymelinked immunosorbent assay (I-ELISA), were evaluated for their efficiency on diagnosis of brucellosis in goats. Serum to antigen ratio, used for RBT and MRBT, which allowed for optimal sensitivity, was 1:1 and 3:1, respectively. CET was performed using microtechnique combined with cold fixation protocol. I-ELISA was performed using conjugated protein G and smooth lipopolysaccharide (sLPS) as antigen with percent positivity (PP) cutoff at 30. Specimens tested included 1,317 goat sera, of which 127 were positive and 1,190 were negative as confirmed by isolation of Brucella melitensis. At 95% of confidence, diagnostic sensitivity (DSe) was 99.2% by RBT,100 % by mRBT, 95.3%by CFT and 99.2% by I-ELISA, while their diagnostic specificity (DSp) was 100% by RBT, mRBT and CFT , and 99.9% by I-ELISA,Comparison of each assay with B. melitensis isolation showed that accuracy (Ac) , kappa (K) value , positive predictive value (PPV) and negative predictive value (NPV) were high. The results of this study showed that RBT, mRBT and I-ELISA were sensitive, specific and suitable for screening , while CFT was suitable for confirmation of Brucella spp. infection in goats. Further study on herd prevalence of brucellosis is needed for the dvelopment of a long-term national prevention and control program of brucellosis in goats.